Home Resources Videos & Webinars SpectraMax M5e Multi-Mode Microplate Reader The SpectraMax M5e Multi. All other trademarks or registered trademarks are the property of their respective owners. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. D. 635. Supplier: Molecular Devices. Molecular Devices. The chamber. 3860 N First Street San Jose,CA 95134 USA +1 408. 3. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. D 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Other technologies such as imaging. Stray light. This page. Figure 3. The M5 has two PMTs. 4. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance X X X X on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Molecular Devices Spectramax M5 Microplate Reader; Related products. Plate chamber design of the SpectraMax category 1 microplate readers. Category 2 and 3 microplate readers use gradient heating as well. SpectraMax® iD5. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Sensitive fluorescent quantitation of DNA with the Quant-iT. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. view more . ] Example of document to evaluate if the reader . The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. SpectraMax Injector Cartridge with SmartInject™ Technology. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax® ABS. These robust readers have been placed in labs from Antarctica to. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. SpectraMax M-series Microplate readers. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 7 are achievable. Fully optimized assays for SpectraMax iD3 reader save time on assay development. SpectraMax M5, Analyst HT or Analyst GT. 1. Ferguson, Ph. FlexStation® 3 Multi-Mode Microplate Reader. spr SpectraMax iD3 SpectraTest ABS2 v1. This document is provided to customers who have purchased Molecular Devices, Inc. 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Cables. l. Prepare your i3/i3x instrument for shipment. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. Add to Cart. SpectraMax Gemini EM & FLEXstation (bottom read) 1. and California) iv SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual. 1 ng/mL to 50 pg/mL (r2 = 1. Plate stacker and robot integration. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence, luminescence, TRF and HTRF. To evaluate the amount of miR-148b mimic released by the retro Diels-Alder reaction (Fig. . Add to Cart. The SpectraTest ABS1 Validation Plate is a comprehensive optical validation package that is com-SpectraMax® Microplate Reader Suite of Products Knowledge Base. Lower end of DNA standard curve. -39. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 800. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 2. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. substrates using the SpectraMax M5 microplate reader and SoftMax® Pro Software. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. 6% for the on-board optics (monochromators). Description 1. Both microplate and cuvette ports can be qualified. spr SpectraMax iD5 SpectraTest ABS2 v1. 1. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Schuler measurements were recorded with a Cary UV-Vis Bio100 dual beam spectrophotometer or Molecular Devices SpectraMax M-series. 0 out of 5 $ 3,799. Molecular Devices has collaborated with various assay partners to optimize andFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. The drawing shows a standard microplate of 15 mm height covered with a lid. Also refer to this Multimode. The bottom read fluorescence mode should be used. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. On-site Repair Service available nationwide for Molecular Devices SpectraMax M5 Plate Reader's. 1). i3. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. 7°C and the bottom to 36. Cavicide (Warehouse, Cat # 79300360) 6. We therefore gathered salient. Cavicide (Warehouse, Cat # 79300360) 6. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. (“Molecular Devices”) equipment, software, reagents, and consumables to use in theWe would like to show you a description here but the site won’t allow us. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. MaterialsThe calculated LOQ values were 0. item 6 Molecular Devices Spectramax M5 Multi-Mode Microplate Reader Parts/Repair Molecular Devices Spectramax M5 Multi-Mode Microplate Reader Parts/Repair $8,000. g. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator 6. SpectraMax Detection Cartridge Fluorescence Polarization SpectraMax® Detection Cartridges offers great sensitivity. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. 1. The colorimetric signal development was stopped after 10 minutes. 315 can't find zero order. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. View the Molecular Devices websiteMolecular Devices’ SpectraMax® L Luminescence Microplate Reader is a dedi-cated reader with an extended dynamic range of more than 8 orders of magnitude. This problem has been remedied by the introduction of Molecular Devices PathCheck Technology, which enables determination of the pathlength in each well of a microplate and automatically normalizes the absorbance value to a 1-cm pathlength. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Failed to load PDF file. com. 100 readings per well gives optimal Z’ values for this assay. Comparing FLIPR Calcium 6 and 6-QF to other dyes shows that both had the largest signal window. 306 plate air cal fail,low light. If depot service is the best option for you we will assist you with logistics to help you keep. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. 1. View the Molecular Devices website. 7. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multi. 1) SpectraMax Glo Steady Luc Reporter Assay (6. and gives specific instructions for using it with SpectraMax® microplate readers and SoftMax Pro. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Table 2. The system provides automation for up to 50 The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Recertification is recommended every two years. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 4. • Software powers discovery on all Molecular Devices readers • Simplified data measurement through library of prewritten protocols. Molecular Devices. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. For a higherMDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. Check software compatibility with Windows 10 and locate your software installer. Histamine H1 is an endogenous receptor in HeLa cells. 0200-0729. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Figure 3. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the. Multimode Readers Comparison Chart. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Optimal instrument. 2. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The following document is intended to demonstrate setup of this instrument. Simple, homogeneous workflow minimizes cell handling. The following document is intended to demonstrate setup of this instrument. The Fluoroblok assays can be read on Spectramax M5/M5e, M4, M3, M2e, Gemini EM, Filtermax and Paradigm microplate readers. Photometric accuracy. 0 mm wide, and heights from 14 mm to 15 mm. The M5 has two PMTs. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. minutes and begin reading microplates with seven mouse clicks. OPTICSon the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. The FlexStation 3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. New applications in minutes Adding modes and functionality is just minutes. 304 signal level saturation. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175) Sunnyvale, CA - May 17, 2011 - Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax® M5e Multi-Mode Microplate Reader for use in NASA's International Space Station; the M5e Reader will be the first microplate reader to be used in space. Oxygen consumption is one of the most informative and direct measures of mitochondrial function. 1. 2), the fluorescence intensity of the FAM conjugated to the miR-148b mimic was measured in each well at 495/520 nm (Excitation/Emission) using a Molecular Devices Spectramax M5 Microplate/Cuvette Reader. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. D 1. Digitizer Systems. It has a cuvette port, spectral scanning, and up to six wavelengths per read. STAKMAX. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. Home Resources Videos & Webinars SpectraMax M5e Multi-Mode Microplate Reader The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. We would like to show you a description here but the site won’t allow us. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. Monday to Friday 8am - 5pm GMT. SpectraMax M5 microplate reader. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. The chamber contains springs that position the cuvette in the proper alignment for a read. Molecular Devices Spectramax M5 Microplate Reader Condition: Used, tested in working condition. Microplate Spectrophotometers. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. 00 shippingdetection in the Molecular Devices SpectraMax M5 device. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Field. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 1. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi-ModeMicroplateReader SpectraMax® SpectraMaxLMicroplateReader For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way? SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). Note: In this user guide, SpectraMax ® refers to several SpectraMax . 3: The cuvette chamber. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The system. UV/Vis Photometric Performance: Wavelength Range. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. Molecular Devices Spectramax M 5 Manual, 2000 Vw Beetle Workshop Manual, Proton Supplier Quality Manual, Beltronics Rx65 Red Manual, Vfr 400 Owners Manual, Hgv Mot Testing Manual, Prostart Manual Installation. #0200-7011) Instrument setupSoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. +44-118-944-8000. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 We would like to show you a description here but the site won’t allow us. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax® M5/M5e Multimode Plate Reader is a high-performance microplate reader that can read volumes as low as 2uL and perform various detection modes for bioresearch and drug discovery applications. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Molecular Devices SpectraMax® M5. 314 low lamp level warning. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Other technologies such as imaging. The StakMax system is an easy-to-set-up, powerful, and reliable stacking solution for. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence, luminescence, TRF and HTRF. The. 9000-0756. Description: Molecular Devices ’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Table of Contents. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Molecular Devices StakMax® Microplate Stacker within . IMAP technology is based on. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Feb 22, 2023By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. ATP standard curves read on SpectraMax microplate readers. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 3 or greater. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Monday to Friday 7am - 5pm Pacific Time. Non-transfected HEK cells were included as controls. moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next-BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. The donor and acceptor can be used to label a variety of biomolecules, for applications. Microplate Reader Shelf. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Sunnyvale, Calif. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the. ©2022 Molecular Devices - All rights reserved. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Molecular Devices’SpectraMax ® Microplate Readers have been validated for Transcreener ADP. LoadingIMAP® Technology from Molecular Devices enables rapid, non-radioactive assay of a wide array of kinases and is suited to both assay development and high-throughput. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. In the M2, M5 and Plus 384, the light. Lower limits of detection for ATP determined using the ATPliteThe Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. 58% for the detection module and 2. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. Modular Multi-Mode Reader also offers user upgradeability so that new detection capabilities can be added as users’ screening needs evolve. This document is provided to customers who have purchased Molecular Devices, Inc. Figure 2 shows the emission spectrum of firefly luciferase obtained on the SpectraMax M5. Preconfigured protocols in SoftMax Pro Software expedite data. SoftMax Pro Software can collect data from one or more microplates orSpectraMax ABS Plus SpectraTest ABS2 v1. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. The following document is intended to demonstrate setup of this instrument and provide representativeMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. All associated StakMax® stackers. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 6. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Depot. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. 2. This integrated system provides users with a multi. Call 516-939-0888 to check current. Wide range of applications; Five preferred detection modes;The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 2. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Home; Knowledge BaseThe introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Monday to Friday 7am - 5pm Pacific Time. Description 1. moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next- BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. The SpectraMax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and well-area scanning. spr SpectraMax Plus 384. HEALTH AND SAFETY CONSIDERAIONS SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. com A. #R7271) Akt1-PKBa 激酶 (Upstate Cat. When the user applies a set temperature of 37°C, the top is heated to 37. Turn on Plate Reader and wait. com A. It does not work as expected. for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. 000). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Download PDF. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. , Sigma cat. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. peptide and phosphopeptide, read on SpectraMax M5 (quadratic curve fit). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 3. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. FlexStation™ I. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. HTRF-compatible SpectraMax Multi-Mode Readers. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. Molecular DevicesSpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. There should be a value for 900 and 1000. Molecular Devices is ISO 17025 accredited† by American Association of Laboratory Accreditation (A2LA), which requires rigorous outside auditing on a regular basis so that you can be assured of the accuracy of your certificate with respect to NIST and NMI traceable standards. 2. Molecular Devices Technical Support. 1. The donor and acceptor can be used to label a variety of biomolecules, for. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. Fluorescence Microplate Reader Comparison Chart. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. D. 2018. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. SpectraMax M5 is ideal for laboratories requiring maximum flexibility in the detection of a. Table of Contents. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 5. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Learn how SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for Molecular Devices' microplate. You should get comparable results from top and bottom reads using EC50's to compare reads. Using a cell-based standard curve and a five-minute incubation, we observed a limit of. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The opaque walls prevent stray light from being detected from neighbouring. +44-118-944-8000. Published Dec 16, 2020 | Updated Dec 16, 2020. 3 mm. Printed in USA. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Materials. Offer Ends In: 18 Days 19: 38: 21. Molecular Devices Spectramax M 5 Manual -. Conventional Electrophysiology. It has a cuvette port, spectral scanning, and up to six wavelengths per read. Anne T. This Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Representative data from three different SpectraMax Quant dsDNA Assay Kits. Figure 2. Published Dec 16, 2020 | Updated Dec 16, 2020. Molecular Devices SpectraMax® series microplate readers. Molecular Devices, LLC Subject: SpectraMax ABS and ABS Plus Microplate Readers are a compact and tunable absorbance microplate readers. The single mode Luminescence Readers (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. 1. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. For life sciences research, the system is well suited. It has patented optimization, IQ/OQ/PM services,. The SpectraMax 190 reader can be integrated with Molecular . 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に.